KEyence Research MIcroscope Testbed
Facility Description
The Keyence BZ-X800E All-In-One Fluorescence Microscope system was delivered to the ISSInternational Space Station in early 2021 and is available for in-orbit imaging.
Specifications:
- Imaging: Fluorescence, brightfield, phase contrast, oblique illumination
- Transmitted light source: 3.7 W LED
- Excitation lamp: 80W metal halide lamp with board wavelength range from UV to IR
- Objective lenes: 2x, 4x, 10x, 20x, 40x, 60x
- Filters: DAPI, GFP, TRITC, Texas Red, Cy5, Polarizer
- Holders: Slide holder (up to three standard 1”x3” slides), well plate holder (standard well plates up to 384 wells), 35 mm dish (standard dishes), versatile and custom holders also available
- XY stage motion range: 114 x 80 mm (4.49” x 3.15”)
- Z stage motion range: 8 mm (0.31”) stroke, minimum 0.1 µm pitch
Observation and Capture Modules:
- Image cytometry module: Capture and analyze several sample areas sequentially in large volumes
- Advanced observation modules
- Z-stack imaging: Capture sample cross sections to render fully focused images of thick samples or create 3D renderings
- Image stitching: Capture large sample areas at high magnification by combining multiple images of a designated sample area into one image
- Sectioning module: Uses structured illumination to create confocal-type images
- Time-lapse module: Capture images at regular time intervals for a specified number of intervals to measure chronological changes to a sample over time (e.g., RGB brightness or morphology changes)
- Video recording module: Record the sample observation area up to 3,600 seconds at a time
Analysis Applications:
- Hybrid cell counting module: Visually separate, count, and measure cells; save and apply counting conditions to multiple images for repeatable results
- Macro cell counting module: Analyze batch images of multiple sample areas simultaneously using specified parameters to generate statistical data such as heat maps, histograms, and bar graphs
- Image stitching: Combine up to 50,000 x 50,000 pixels into a single image
- Measurement module: Measure distance, radius, angles, multipoint line length, circle or free-form area
- 3D application module: Transform Z-stack images into 3D renderings and XYZ cross-sectional views
- Motion tracking module: Automatically tracks a selected sample target to measure travel range, speed, and movement over time
Current Status: Onboard
Availability: Please contact the facility manager
ISS Environment: Internal
Owner: NASA
Operator/Implementation Partner:
Leidos Innovations Corporation
Developer(s):
Keyence Corporation
Facility Manager:
William Therrien
Leidos Innovations Corporation
Manager Email:
william.g.therrien@leidos.com
Parent Facility: MSRR-1
Child Facility:
Sponsoring Space Agency: NASA
Equipment Category: Capability
Additional Information: